0613 GMT September 23, 2020
Putin stressed it was inappropriate to make "unsubstantiated accusations based on nothing against the Russian side," according to a Kremlin readout of the call, AFP reported.
Putin said that Russia wanted Germany to hand over medical test results taken from Navalny in order to clarify the real circumstances of the incident.
French and Swedish laboratories earlier confirmed German findings that Navalny was poisoned with a Novichok nerve agent.
Macron urged Putin to urgently shed light on the incident, the French presidency said in a statement.
Macron told Putin that it is "imperative that all light be shed, without delay, on the circumstances of this attempted murder and who is responsible," the Elysee said.
He also informed Putin that France's own analysis had confirmed Germany's conclusion that Navalny was poisoned by a Novichok nerve agent "in contravention of international norms on using chemical weapons."
Germany has accused Russia of trying to poison Navalny and demanded an explanation from Moscow for what it said was the banned Soviet-era nerve agent Novichok found in his body by German doctors, according to Reuters.
Navalny was flown to Germany last month after falling violently ill while traveling in Siberia.
Moscow has insisted it has seen no evidence he was poisoned and earlier on Friday said it would ask to send investigators to Berlin in response to the German demands.
According to the Elysee, Macron on Monday "expressed his full solidarity with Germany over the steps to be taken and the consequences to be drawn from the situation."
"Clarification is required from Russia as part of a credible and transparent investigation," he added.