"I have no doubt that the perseverance of the Iranian people during these past three years will force the new US government to succumb and resume its commitments," Rouhani said in televised remarks in a ceremony held to inaugurate a number of Interior Ministry’s development projects.
"The sanctions will be broken," he added.
Tensions between Tehran and Washington soared during Trump's presidency, especially after 2018 when he withdrew Washington from the Joint Comprehensive Plan of Action (JCPOA) and reimposed unilateral sanctions on Iran, AFP wrote.
Biden, who defeated Trump at the ballot box in November, has signaled a willingness to return to diplomacy with Iran.
Iran’s government has already signaled its openness to the incoming US administration and called on Washington to return to the nuclear accord and lift sanctions.
Rouhani’s comments come a day after Iran's Leader Ayatollah Seyyed Ali Khamenei said that Iran should bolster itself to "nullify" the effects of the sanctions imposed by the Trump administration, but should "not delay for even an hour" in case they can be lifted.
Rouhani echoed the Leader, saying time should not be wasted.
"We must act in order to nullify the effects of the sanctions... as the Leader has said."
"We should not wait, not even one hour, for the lifting of the sanctions. The government must do everything in its power to break the sanctions," Rouhani added.
He said Iran will do "everything possible to achieve" what he described as a "very important instruction" made by the Leader.